I think Raj mentioned the Sailor Moon incident even before that. Maybe in the Vegas episode? He said something about calling her.

When Nolan intercepted Conrad's call, Aiden says something like, "I guess I'm going to the morgue." So when Conrad called for back up, they threatened him with "Charlotte's" ear.

I loved that after 2+ years, Emily finally seems to have a measure of her revenge on the Graysons. What I don't understand is why she couldn't have done this 2 years ago? What made it only possible NOW to threaten the one family member that both Victoria and Conrad hold dear? It seemed to work like a charm.